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Separation of Spliceosome Assembly from Catalysis with Caged pre‐mRNA Substrates
Author(s) -
Chaulk Steven G.,
MacMillan Andrew M.
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010601)40:11<2149::aid-anie2149>3.0.co;2-z
Subject(s) - spliceosome , rna splicing , chemistry , catalysis , rna , substrate (aquarium) , messenger rna , precursor mrna , biophysics , biochemistry , biology , gene , ecology
Getting spliced? Pre‐mRNA splicing is catalyzed by the spliceosome, a complex assembly of proteins and RNA, which forms in an ordered fashion on the substrate. If one of the residues of the substrate is caged with a photolabile o ‐nitrobenzyl group, the splicing reaction can be transiently blocked, until subsequent initiation by photolysis of the complexes (see scheme). This RNA‐caging approach effectively separates the spliceosome assembly from the catalytic reaction and allows the two processes to be studied independently.