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A Ligand‐Modulated Padlock Oligonucleotide for Supercoiled Plasmids
Author(s) -
Roulon Thibaut,
Hélène Claude,
Escudé Christophe
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010417)40:8<1523::aid-anie1523>3.0.co;2-a
Subject(s) - plasmid , triple helix , oligonucleotide , catenation , ligand (biochemistry) , dna supercoil , supramolecular chemistry , chemistry , helix (gastropod) , biophysics , nanotechnology , dna , crystallography , stereochemistry , biology , materials science , dna replication , biochemistry , receptor , crystal structure , ecology , snail
Catenation of a circular oligonucleotide to a supercoiled plasmid can be achieved in high yields by means of ligand‐induced triple‐helix formation. The noncovalent interactions in this supramolecular structure can be modulated by a triplex‐stabilizing agent (TSA; see picture). The circular oligonucleotide represents a noncovalent anchor for plasmid functionalization.