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Analysis of the Topology of the Chromophore Binding Pocket of Phytochromes by Variation of the Chromophore Substitution Pattern
Author(s) -
Robben Uwe,
Lindner Ingo,
Gärtner Wolfgang,
Schaffner Kurt
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010316)40:6<1048::aid-anie10480>3.0.co;2-9
Subject(s) - chromophore , steric effects , phytochrome , substituent , thermal stability , chemistry , stereochemistry , alkyl , covalent bond , derivative (finance) , photochemistry , biology , organic chemistry , red light , botany , financial economics , economics
The assembly kinetics of open‐chain tetrapyrroles in a recombinant apophytochrome and the thermal reversal of the P fr to the P r form of the phytochrome are strongly affected by steric interactions between the protein and the chromophore. Relative to the chromoprotein with a naturally occurring chromophore ( 1 ), bulkier alkyl groups on ring D regioselectively retard the incorporation and covalent binding (whereby C17 derivatives, particularly the 17‐CH(CH 3 ) 2 derivative 2 , have a larger effect than C18 derivatives), and either decrease or increase the thermal stability of the P fr form with increasing substituent size at C17/C18, respectively (the 18‐C(CH 3 ) 3 derivative 3 shows the largest effect here).