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Synthesis of an Array Comprising 837 Variants of the hYAP WW Protein Domain
Author(s) -
Toepert Florian,
Pires José R.,
Landgraf Christiane,
Oschkinat Hartmut,
SchneiderMergener Jens
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010302)40:5<897::aid-anie897>3.0.co;2-x
Subject(s) - schematic , amino acid , chemistry , domain (mathematical analysis) , combinatorial chemistry , stereochemistry , biochemistry , electronic engineering , engineering , mathematical analysis , mathematics
Comprehensive structure–function analyses are possible with an array comprising more than 800 synthetic variants of a protein domain composed of 44 amino acids. This array was produced by SPOT synthesis on a cellulose membrane (see schematic representation) and successfully employed for a parallel ligand‐binding assay.