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Parallel Reactions for Enantiomeric Quantification of Peptides by Mass Spectrometry
Author(s) -
Tao W. Andy,
Cooks R. Graham
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010216)40:4<757::aid-anie7570>3.0.co;2-h
Subject(s) - diastereomer , chemistry , analyte , enantiomer , mass spectrometry , dissociation (chemistry) , methanol , molecule , ion , chromatography , tandem mass spectrometry , computational chemistry , organic chemistry
Independent parallel ion/molecule reactions and fast dissociation have been used to quantify small enantiomeric excesses of dipeptides in a new mass‐spectrometric method. The ee values are determined by simply mixing the analyte with CuCl 2 and a chiral reference compound (ref*) in aqueous methanol and observing the relative abundances of the diastereomeric cluster ions (see scheme).