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The First Direct Determination of a Ligand Binding Constant in Protein Crystals
Author(s) -
Wu Suying,
Dornan Jacqueline,
Kontopidis George,
Taylor Paul,
Walkinshaw Malcolm D.
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010202)40:3<582::aid-anie582>3.0.co;2-0
Subject(s) - ligand (biochemistry) , crystallography , chemistry , binding constant , constant (computer programming) , protein ligand , binding site , biochemistry , receptor , computer science , programming language
Crystallographic snapshots of protein–ligand complexes have been determined by soaking protein crystals in solutions of different ligand concentrations. The crystallographic refinement of ligand occupancy allows the calculation of the ligand binding constant in the crystal. The picture shows three different stages along the binding pathway: from free protein (left) to ligand‐bound protein (right).