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Mass Spectrometry of an Intact Virus
Author(s) -
Fuerstenau Stephen D.,
Benner W. Henry,
Thomas John J.,
Brugidou Christophe,
Bothner Brian,
Siuzdak Gary
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010202)40:3<541::aid-anie541>3.0.co;2-k
Subject(s) - virus , rna , mottle , mass spectrometry , tobacco mosaic virus , virology , homogeneous , chemistry , rna virus , icosahedral symmetry , cowpea mosaic virus , plant virus , turnip yellow mosaic virus , biology , analytical chemistry (journal) , physics , chromatography , crystallography , biochemistry , gene , thermodynamics
The simultaneous measurement of the time‐of‐flight and the number of charges on each electrosprayed ion provides a new way to determine the mass of a whole intact virus. The weights of an icosahedral virus (rice yellow mottle virus) consisting of a single‐stranded RNA surrounded by a homogeneous protein shell with a mass of 6.5×10 6 Da, and a rod‐shaped RNA virus (tobacco mosaic virus) with a mass of 40.5×10 6 Da were measured with this technique.