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β ‐Mannosynthase: Synthesis of β ‐Mannosides with a Mutant β ‐Mannosidase
Author(s) -
Nashiru Oyekanmi,
Zechel David L.,
Stoll Dominik,
Mohammadzadeh Taraneh,
Warren R. Antony J.,
Withers Stephen G.
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010119)40:2<417::aid-anie417>3.0.co;2-v
Subject(s) - mannosidase , mutant , chemistry , biochemistry , mannose , gene
Engineering enzymes : The glutamic acid nucleophile of a retaining β ‐mannosidase has been replaced with a serine residue to form a β ‐mannosynthase. When the new enzyme is provided with an α ‐mannosyl fluoride donor and an appropriate acceptor, β ‐mannoside linkages are synthesized (see scheme). Remarkably, α ‐mannosyl fluoride can be generated in situ by providing the mannosynthase with excess fluoride ion.