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Visualization of Single Multivalent Receptor–Ligand Complexes by Transmission Electron Microscopy
Author(s) -
Gestwicki Jason E.,
Strong Laura E.,
Kiessling Laura L.
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20001215)39:24<4567::aid-anie4567>3.0.co;2-f
Subject(s) - ligand (biochemistry) , valency , chemistry , transmission electron microscopy , receptor , concanavalin a , conjugated system , biophysics , crystallography , stereochemistry , combinatorial chemistry , nanotechnology , materials science , polymer , biochemistry , biology , organic chemistry , philosophy , linguistics , in vitro
Gold nanoparticle labels enable the position of receptors (concanavalin A) bound to multivalent ligands (see picture; top: straptavidin‐conjugated gold particles, middle: receptors, bottom: ligand) assembled using the ring‐opening metathesis polymerization to be determined by transmission electron microscopy. The number of concanavalin A tetramers incorporated into the receptor–ligand complexes depends on the ligand valency. The results illustrate a general approach for the elucidation of the stoichiometry of individual multivalent ligand–receptor complexes, which is critical for understanding the mechanism of multivalent binding events.