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A New Self‐Assembling System for Targeted Gene Delivery
Author(s) -
Eaton Michael A. W.,
Baker Terence S.,
Catterall Catherine F.,
Crook Kenneth,
Macaulay Graham S.,
Mason Barbara,
Norman Timothy J.,
Parker David,
Perry Justin J. B.,
Taylor Richard J.,
Turner Alison,
Weir A. Neil
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20001117)39:22<4063::aid-anie4063>3.0.co;2-x
Subject(s) - gene delivery , computational biology , nanotechnology , gene , genetic enhancement , computer science , biology , genetics , materials science
Polyamine‐containing bolaamphiphiles bind and compact DNA to form well‐defined assemblies that resist nuclease attack and efficiently transfect Chinese hamster ovary cells. Nonspecific uptake is reduced by the incorporation of a low percentage of polyethylene glycol bolaamphiphile and the targeted transfection of a human T cell line has been demonstrated using an internalizing anti‐CD3‐antibody/bolaamphiphile conjugate incorporated at 0.004 % into the supramolecular array.