Premium
Light‐Driven Microfabrication: Assembly of Multicomponent, Three‐Dimensional Structures by Using Optical Tweezers
Author(s) -
Holmlin R. Erik,
Schiavoni Michele,
Chen Clifford Y.,
Smith Stephen P.,
Prentiss Mara G.,
Whitesides George M.
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20001002)39:19<3503::aid-anie3503>3.0.co;2-m
Subject(s) - microfabrication , optical tweezers , wheat germ agglutinin , nanotechnology , materials science , chemistry , lectin , optics , biochemistry , physics , medicine , alternative medicine , pathology , fabrication
Optical tweezers were used to fabricate two‐ and three‐dimensional microstructures incorporating cells and microspheres (see schematic representation). The microspheres were covalently linked to wheat germ agglutinin; this lectin adhered biospecifically to N ‐acetyl glucosamine and N ‐acetyl neuraminic acid residues of the cell‐surface oligosaccharides, and provided the links connecting the cells. This method of microfabrication provides an approach to generate well‐defined arrays of cells and has the potential to be useful in areas where aggregates of cells are relevant.