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Purification and Partial Characterization of Tryptophan 7‐Halogenase (PrnA) from Pseudomonas fluorescens
Author(s) -
Keller Sascha,
Wage Tobias,
Hohaus Kathrin,
Hölzer Manuela,
Eichhorn Eric,
van Pée KarlHeinz
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20000703)39:13<2300::aid-anie2300>3.0.co;2-i
Subject(s) - tryptophan , flavin group , chemistry , yield (engineering) , biochemistry , enzyme , amino acid , materials science , metallurgy
In an oxygen‐dependant reaction , tryptophan 7‐halogenase (PrnA) regioselectively chlorinates tryptophan ( 1 ) at the 7‐position to yield 2 . During purification of the halogenase, a flavin reductase, as second protein component, was detected in addition to NADH and FAD; this second protein is also required for the reaction.