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Expanding the Scope of Protein Biosynthesis by Altering the Methionyl‐tRNA Synthetase Activity of a Bacterial Expression Host
Author(s) -
Kiick Kristi L.,
van Hest Jan C. M.,
Tirrell David A.
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20000616)39:12<2148::aid-anie2148>3.0.co;2-7
Subject(s) - transfer rna , aminoacyl trna synthetase , amino acid , biochemistry , protein biosynthesis , biosynthesis , chemistry , host (biology) , bacteria , biology , enzyme , gene , rna , genetics
The incorporation of amino acids into proteins in vivo is controlled by the aminoacyl‐tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans ‐crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl‐tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.