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A Novel Bovine β ‐1,4‐Galactosyltransferase Reaction To Yield β ‐ D ‐Galactopyranosyl‐(1‐3)‐Linked Disaccharides from L ‐Sugars
Author(s) -
Nishida Yoshihiro,
Tamakoshi Hideaki,
Kitagawa Yuki,
Kobayashi Kazukiyo,
Thiem Joachim
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20000602)39:11<2000::aid-anie2000>3.0.co;2-3
Subject(s) - disaccharide , galactosyltransferase , chemistry , xylose , yield (engineering) , biocatalysis , acceptor , sugar , galactose , stereochemistry , biochemistry , organic chemistry , enzyme , catalysis , reaction mechanism , materials science , metallurgy , physics , fermentation , condensed matter physics
L ‐Glucose and L ‐xylose are not acceptor substrates for bovine β ‐1,4‐galactosyltransferase, but their β ‐1‐NAc derivatives are moderate substrates. The enzymatic reaction gives an unnatural β ‐1,3‐linked disaccharide which carries the corresponding L ‐sugar‐1‐NAc at the reducing terminal. In the case of L ‐glucose‐1‐NAc, the disaccharide is β ‐ d‐ Gal‐(1‐3)‐ β ‐ L ‐Glc‐1‐NAc ( 1 ).