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Oligothiophene Isothiocyanates as Fluorescent Markers
Author(s) -
Barbarella Giovanna
Publication year - 2002
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20021115)8:22<5072::aid-chem5072>3.0.co;2-m
Subject(s) - biomolecule , surface modification , fluorescence , covalent bond , chemistry , conjugate , combinatorial chemistry , thiophene , molecule , isothiocyanate , photochemistry , solubility , polymer , small molecule , organic chemistry , biochemistry , mathematical analysis , physics , mathematics , quantum mechanics
Thiophene oligomers have been studied so far mainly for their semiconductor and charge‐transport properties. However, these compounds are also highly fluorescent and soluble. Solubility and fluorescence frequencies and efficiencies can be tailored by means of appropriate functionalization of the aromatic backbone. Functionalization with the isothiocyanate group (NCS) allows these molecules to form covalent bonds with NH 2 ‐containing biomolecules and give rise to optically and chemically very stable fluorescent bioconjugates. Examples of photostable conjugates formed with monoclonal antibodies are reported.