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Chemical Approaches Untangling Sequence‐Specific DNA Binding by Proteins
Author(s) -
Sato Shinichi,
Hagihara Masaki,
Sugimoto Kenji,
Morii Takashi
Publication year - 2002
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20021115)8:22<5066::aid-chem5066>3.0.co;2-0
Subject(s) - dna , computational biology , sequence (biology) , dna sequencing , peptide , chemistry , dna binding protein , biochemistry , biology , gene , transcription factor
Structure‐based design of novel DNA‐binding proteins provides an ultimate test of our understanding of protein–DNA interactions. A combination of synthetic, organic, biochemical and molecular biological approaches has been developed to study the principle of molecular recognition associated with the protein–DNA interactions. The strategies enabled a specific formation of noncovalent peptide dimers and determination of the preferential DNA‐binding sequence of short peptides.