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Tag‐Reporter and Resin Capture–Release Strategy in Oligosaccharide Synthesis
Author(s) -
Ito Yukishige,
Manabe Shino
Publication year - 2002
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20020715)8:14<3076::aid-chem3076>3.0.co;2-n
Subject(s) - oligosaccharide , chemistry , ethylene glycol , glycosylation , cysteine , peg ratio , ethylene , chromatography , combinatorial chemistry , organic chemistry , biochemistry , catalysis , finance , economics , enzyme
Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a “tag” in oligosaccharide synthesis due to its high polarity. Real‐time reaction monitoring was achieved by use of MALDI‐TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine‐supported insoluble resin enables the purification of the chloroacetyl‐bound compounds on soluble PEG.