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Selective Adsorption of Biopolymers on Zeolites
Author(s) -
Matsui Masayoshi,
Kiyozumi Yoshimichi,
Yamamoto Taichi,
Mizushina Yoshiyuki,
Mizukami Fujio,
Sakaguchi Kengo
Publication year - 2001
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20010401)7:7<1555::aid-chem1555>3.0.co;2-o
Subject(s) - isoelectric point , adsorption , van der waals force , chemistry , mesoporous material , hydrophobic effect , zeolite , chemical engineering , molecule , ion exchange , organic chemistry , chromatography , crystallography , ion , catalysis , engineering , enzyme
Zeolites adsorb biopolymers on their surface and may be suitable as a new type of chromatographic carrier material for proteins, nucleic acids, and their conjugates. We report here various parameters that influence the adsorption of biopolymers on synthesized zeolites with regard to the Si/Al 2 ratio and three‐dimensional structure. There are three physicochemical principles that may underly the adsorption: 1) below the isoelectric point (pI), mainly Coulombic attraction similar to ion‐exchange chromatography; 2) at pI, hydrophobic interactions (a kind of van der Waals attraction) plus the three‐dimensional mesopore structure; and 3) above pI, the sum of the Coulombic repulsion and attraction forces, such as the hydrophobic interaction, and also substitution reaction of water on the Al molecule with a protein amino‐base. At high Si/Al 2 ratio in the presence of a small amount of Al and with mesopores between the zeolite particles, maximal adsorption was seen at pI and was suggested to be dependent on the number of hydrophobic interaction points on the mesopores, and their morphology. The application of zeolites to biochemistry and biotechnology is also discussed.