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Selective Adhesion of Endothelial Cells to Artificial Membranes with a Synthetic RGD‐Lipopeptide
Author(s) -
MarchiArtzner Valérie,
Lorz Barbara,
Hellerer Ulrike,
Kantlehner Martin,
Kessler Horst,
Sackmann Erich
Publication year - 2001
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/1521-3765(20010302)7:5<1095::aid-chem1095>3.0.co;2-b
Subject(s) - lipopeptide , adhesion , membrane , liposome , vesicle , biophysics , peptide , artificial cell , chemistry , cell adhesion , lipid bilayer fusion , microbiology and biotechnology , biochemistry , biology , bacteria , genetics , organic chemistry
A constrained cyclic Arg‐Gly‐Asp‐ D ‐Phe‐Lys, abbreviated as cyclo(‐RGDfK‐), lipopeptide has been synthesized and incorporated into artificial membranes such as giant vesicles with DOPC and solid‐supported lipid bilayers. The selective adhesion and spreading of endothelial cells of the human umbilical cord on solids functionalized by membranes with this RGD‐lipopeptide have been observed. Furthermore, we have demonstrated strong selective adhesion of giant vesicles to endothelial cells through local adhesion domains by combined application of hydrodynamic flow field and reflection interference contrast microscopy (RICM). The adhesion can be inhibited by competition with a water‐soluble RGD peptide. We suggest that this strategy could improve the efficiency of liposomes targeting used as vectors or as drug carriers to cells.