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Hybrid vector designs to control the delivery, fate and expression of transgenes
Author(s) -
Lam Paula Y. P.,
Breakefield Xandra O.
Publication year - 2000
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/1521-2254(200011/12)2:6<395::aid-jgm146>3.0.co;2-k
Subject(s) - transgene , transduction (biophysics) , biology , gene delivery , vector (molecular biology) , viral vector , viral replication , microbiology and biotechnology , gene , genetic enhancement , computational biology , gene expression , replication (statistics) , mutant , virus , virology , genetics , recombinant dna , biochemistry
One of the greatest challenges to gene therapy is the targetting of gene delivery selectively to the sites of disease and regulation of transgene expression without adverse effects. Ultimately, the successful realization of these goals is dependent upon improvements in vector design. Over the years, viral vector design has progressed from various types of replication‐defective viral mutants to replication‐conditioned viruses and, more recently, to ‘gutted’ and hybrid vectors, which have, respectively, eliminated expression of non‐relevant or toxic viral genes and incorporated desired elements of different viruses so as to increase the efficacy of gene delivery in vivo . This review will focus on the different viral and cellular elements which have been incorporated into virus vectors to: improve transduction efficiencies; alter the entry specificity of virions; control the fate of transgenes in the host cells; and regulate transgene expression. Copyright © 2000 John Wiley & Sons, Ltd.