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Extremely low frequency electromagnetic fields and heat shock can increase microvesicle motility in astrocytes
Author(s) -
Gölfert F.,
Hofer A.,
Thümmler M.,
Bauer H.,
Funk R.H.W.
Publication year - 2001
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/1521-186x(200102)22:2<71::aid-bem1008>3.0.co;2-i
Subject(s) - microvesicle , microvesicles , motility , mechanosensitive channels , medicine , chemistry , biology , microbiology and biotechnology , biochemistry , microrna , receptor , ion channel , gene
The effect of extremely low frequency electromagnetic fields (EMF) on microvesicles was examined in rat astrocytes by video‐enhanced microscopy in combination with a perfusable cell chamber. The EMF effect was compared with the effect of heat shock (HS) and with a combination of them both. The velocity of microvesicles was measured using image processing software (NIH Scion image 1.61). After exposure of astrocytes to EMF (50 Hz, 100μT, 1 h), the velocity of microvesicles in astrocytes increased from 0.32 ± 0.03 μm/s (n = 120, 95% CI) in the untreated control group to 0.41 ± 0.03 μm/s (n = 175, 95% CI). Fifteen minutes after HS (45°C, 10 min) the microvesicles showed a velocity of 0.56 ± 0.03 μm/s (n = 125, 95% CI). Combination of HS and EMF led to an increase in velocity up to 0.54 ± 0.03 μm/s (n = 110, 95% CI). No significant difference between HS and HS+EMF was found. Compared to the untreated control group, the increased microvesicle velocity of the exposed cells might be a stress response of the cell. It is possibly a sign of intensified intracellular traffic required to adjust the metabolic needs. Bioelectromagnetics 22:71–78, 2001. © 2001 Wiley‐Liss, Inc.