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Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields
Author(s) -
Shckorbatov Yury G.,
Shakhbazov Valery G.,
Rudenko Andrey O.
Publication year - 2001
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/1521-186x(200102)22:2<106::aid-bem1013>3.0.co;2-2
Subject(s) - electrokinetic phenomena , buccal administration , electric field , biophysics , chemistry , nanotechnology , materials science , physics , biology , dentistry , medicine , quantum mechanics
The influence of an alternating (50 Hz) electric field (5–110 V/cm) on the state of human buccal epithelium cells was studied by the methods of intracellular microelectrophoresis, heterochromatin staining with orcein, and indigo carmine staining for viability and membrane integrity evaluations. Electric field exposure induced an increase in electrophoretic mobility of cell nuclei, decreased numbers of heterochromatin granules near the inner membrane of cell nucleus, and induced cell membrane damage; but cell viability was conserved. Nuclear and cell membrane properties varied with electric field strength and age of the donors. The data obtained are interpreted as evidence of electric field induced activation of the functional state of nuclei. Bioelectromagnetics 22:106–111, 2001. © 2001 Wiley‐Liss, Inc.