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HPLC enantiomeric resolution of novel aromatase inhibitors on cellulose‐ and amylose‐based chiral stationary phases under reversed phase mode
Author(s) -
AboulEnein Hassan Y.,
Ali Imran,
Gübitz Gerald,
Simons Claire,
Nicholls Paul J.
Publication year - 2000
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/1520-636x(2000)12:10<727::aid-chir5>3.0.co;2-t
Subject(s) - chemistry , enantiomer , cellulose , tris , chromatography , aromatase , high performance liquid chromatography , tetrafluoroborate , carbamate , thiazole , stereochemistry , medicinal chemistry , organic chemistry , ionic liquid , catalysis , medicine , biochemistry , cancer , breast cancer
The chiral resolution of seven aromatase inhibitors (four triazole derivatives (Ia, Ib, Ic, and Id) and three tetrazole derivatives (IIa, IIb, and IIc)) was achieved on Chiralcel OJ‐R [cellulose tris (4‐methyl benzoate)], Chiralcel OD‐RH [cellulose tris (3,5‐dimethylphenyl carbamate)], and Chiralpak AD‐RH [amylose tris (3,5‐dimethylphenyl carbamate)] chiral stationary phases. The mobile phases used were A: 2‐PrOH‐MeCN (90:10, v/v); B: 2‐PrOH‐MeCN (50:50, v/v); C: MeCN‐H 2 O (50:50, v/v); D: MeCN‐H 2 O (80:20, v/v); and E: MeCN‐H 2 O (95:05, v/v). The flow rate was 0.5 mL/min for all the mobile phases. The resolution capability of these chiral stationary phases were in the order Chiralpak AD‐RH > Chiralcel OD‐RH > Chiralcel OJ‐R. The values of α and Rs of the resolved enantiomers of the aromatase inhibitors varied from 1.02–5.63 and 1.12–6.72, respectively. Chirality 12:727–733, 2000. © 2000 Wiley‐Liss, Inc.