Premium
Involvement of adipokinetic hormone in the homeostatic control of hemolymph trehalose concentration in the larvae of Bombyx mori
Author(s) -
Oda Y.,
Uejuma M.,
Iwami M.,
Sakurai S.
Publication year - 2000
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/1520-6327(200012)45:4<156::aid-arch3>3.0.co;2-e
Subject(s) - hemolymph , trehalose , adipokinetic hormone , biology , homeostasis , instar , bombyx mori , larva , biochemistry , medicine , endocrinology , fat body , botany , gene
Prior to wandering, 5th instar larvae of the silkworm, Bombyx mori , maintain constant hemolymph titers of trehalose. Head ligation of day 3, 5th instar larvae significantly decreased the hemolymph trehalose concentrations, but the concentrations did not decrease in starved larvae. After being diluted by replacement of larval hemolymph with insect Ringer’s solution, the trehalose concentrations recovered the initial levels in 90 min in the non‐ligated larvae, while they were not restored in 90 min in the neck‐ligated larvae. These results suggest that a head factor(s) with hypertrehalosemic activity is involved in the homeostatic control of hemolymph trehalose concentration. When adipokinetic hormone (AKH) was injected into neck‐ligated larvae, the trehalose concentrations increased in 2 h and decreased thereafter. Repeated injections of AKH every 4 h maintained the concentrations for 12 h. These findings suggest that AKH induces a hypertrehalosemic response and is involved in the homeostasis of hemolymph trehalose concentration in the larval feeding period. Arch. Insect Biochem. Physiol. 45:156–165, 2000. © 2001 Wiley‐Liss, Inc.