Premium
c‐Fos‐related antigens in the central nervous system of an insect, Acheta domesticus
Author(s) -
Renucci Marielle,
Tirard Alain,
Charpin Pierre,
Augier Roger,
Strambi Alain
Publication year - 2000
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/1520-6327(200012)45:4<139::aid-arch1>3.0.co;2-n
Subject(s) - acheta , biology , juvenile hormone , endocrinology , medicine , ecdysteroid , immunostaining , western blot , ecdysone , antigen , microbiology and biotechnology , hormone , immunohistochemistry , immunology , cricket , biochemistry , zoology , gene
Fos‐related antigens (Fra) were detected in the nuclei of neurones in young adult Acheta domesticus female crickets by immunohistochemical analysis, using an antibody that recognizes the amino‐acid sequence 127–152 of c‐Fos protein. Specificity of Fra immunoreactivity was confirmed by Western blot analysis of nuclear extracts from neural tissues. A major immunoreactive doublet with an apparent molecular mass of 52,000/54,000 Da was detected in nuclear extracts. Immunostaining of the 52,000/54,000 Da doublet showed variations in intensity during the first 5 days following the imaginal molt. Staining was more intense between day 2 and day 4 when ecdysteroid titers were high. Expression of Fra was low in allatectomized (i.e., deprived of juvenile hormone and ecdysteroids) and ovariectomized (i.e., deprived of ecdysteroids) females as compared to control females. These results show the involvement of hormone‐regulated process in expression of Fra. The effect of nociceptive stimulation on Fra expression was tested. Twenty minutes after removal of the ovipositor, a supplementary band with an apparent molecular mass of 70,000 Da appeared in the nuclear extracts, then decreased and disappeared totally after 45 min. Several other Fos‐related antigens with different temporal patterns of expression were also detected. Arch. Insect Biochem. Physiol. 45:139–148, 2000. © 2001 Wiley‐Liss, Inc.