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Aedes aegypti TMOF modulates ecdysteroid production by prothoracic glands of the gypsy moth, Lymantria dispar
Author(s) -
Gelman Dale B.,
Borovsky Dov
Publication year - 2000
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/1520-6327(200010)45:2<60::aid-arch2>3.0.co;2-f
Subject(s) - lymantria dispar , gypsy moth , ecdysteroid , aedes aegypti , biology , prothoracic gland , dispar , medicine , lepidoptera genitalia , zoology , endocrinology , ecdysone , ecology , larva , microbiology and biotechnology , hormone , entamoeba histolytica
Abstract Trypsin modulating oostatic factor (TMOF) is a decapeptide that inhibits the biosynthesis of trypsin‐like enzymes in the midgut of several insect species and, as such, serves as a dipteran oostatic hormone. In vitro incubation of lepidopteran prothoracic glands with Aedes aegypti TMOF revealed that this decapeptide, in the presence of brain extract, modulates ecdysteroid production. The modulatory effect was highly dependent on both the concentration of TMOF and brain extract. Typically, TMOF was stimulatory in the presence of lower concentrations of Lymantria dispar brain extract (0.01 and 0.025 brain equivalent), and either neutral or inhibitory at higher concentrations (0.25, 0.5, and 1.0 brain equivalent) of extract. In the presence of European corn borer ( Ostrinia nubilalis ) brain extract, TMOF also exhibited modulatory effects, effects that again were dependent on the concentrations of both brain extract and TMOF present in the incubation medium. At 1.5 brain equivalents, TMOF was inhibitory at all but the highest concentration tested (5×;10 –6 M), at 1.0 brain equivalent, TMOF was stimulatory at 10 –6 M and at 0.5 brain equivalents, TMOF did not significantly affect PTG synthesis of ecdysteroids. Results suggest the presence of a modulatory peptide(s), which fine tunes the synthesis and release of ecdysteroids by PTGs in accordance with the insect’s developmental/physiological requirements. Arch. Insect Biochem. Physiol. 45:60–68, 2000. Published 2000 Wiley‐Liss, Inc.