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Simultaneous Immobilization of Horseradish Peroxidase and Glucose Oxidase in Mesoporous Sol–Gel Host Materials
Author(s) -
Wei Yen,
Dong Hua,
Xu Jigeng,
Feng Qiuwei
Publication year - 2002
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/1439-7641(20020916)3:9<802::aid-cphc802>3.0.co;2-h
Subject(s) - glucose oxidase , horseradish peroxidase , mesoporous material , peroxidase , substrate (aquarium) , microporous material , chemistry , immobilized enzyme , enzyme , chemical engineering , nuclear chemistry , nanotechnology , biochemistry , organic chemistry , materials science , catalysis , oceanography , engineering , geology
One‐step simultaneous immobilization of two enzymes , horseradish peroxidase (HRP) and glucose oxidase (GOx), was achieved in mesoporous materials. β ‐ D ‐Glucose was the substrate of GOx to yield H 2 O 2 , which became the substrate of the second enzyme, HRP. The mesoporous biogels showed two to three orders of magnitude faster response to β ‐ D ‐glucose and greater enzymatic activity than those of conventional microporous biogels.