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Two‐Photon Fluorescence Cross‐Correlation Spectroscopy
Author(s) -
Schwille Petra,
Heinze Katrin G.
Publication year - 2001
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/1439-7641(20010518)2:5<269::aid-cphc269>3.0.co;2-y
Subject(s) - spectroscopy , fluorescence correlation spectroscopy , fluorescence , excitation , molecule , fluorescence spectroscopy , fluorescence cross correlation spectroscopy , two photon excitation microscopy , excitation wavelength , photon , chemistry , molecular physics , wavelength , materials science , atomic physics , physics , optics , optoelectronics , organic chemistry , quantum mechanics
Dual‐color cross‐correlation spectroscopy is a special kind of fluctuation analysis which selectively probes the formation or deletion of linkages between two different fluorescently labeled molecules at extremely low concentrations. Two‐photon excitation can, under certain circumstances, significantly simplify this method if different probe molecules with distinct emission properties are accessible by a common IR excitation wavelength.