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Novel Paromamine Derivatives Exploring Shallow‐Groove Recognition of Ribosomal‐ Decoding‐Site RNA
Author(s) -
Simonsen Klaus B.,
Ayida Benjamin K.,
Vourloumis Dionisios,
Takahashi Masayuki,
Winters Geoffrey C.,
Barluenga Sofia,
Qamar Seema,
Shandrick Sarah,
Zhao Qiang,
Hermann Thomas
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20021202)3:12<1223::aid-cbic1223>3.0.co;2-w
Subject(s) - rna , ribosomal rna , transcription (linguistics) , ribosome , chemistry , computational biology , biology , microbiology and biotechnology , biochemistry , gene , philosophy , linguistics
Natural aminoglycoside antibiotics recognize an internal loop of bacterial ribosomal‐decoding‐site RNA by binding to the deep groove of the RNA structure. We have designed, synthesized, and tested RNA‐targeted paromamine derivatives that exploit additional interactions on the shallow groove face of the decoding‐site RNA. An in vitro transcription–translation assay of a series of 6′‐derivatives showed the 6′‐position to be very sensitive to substitution. This result suggests that the group at the 6′‐position plays a pivotal role in RNA target recognition.