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A Non‐Antibacterial Oxazolidinone Derivative that Inhibits Epithelial Cell Sheet Migration
Author(s) -
Mc Henry Kevin T.,
Ankala Sudha V.,
Ghosh Arun K.,
Fenteany Gabriel
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20021104)3:11<1105::aid-cbic1105>3.0.co;2-s
Subject(s) - motility , cell growth , cell migration , chemistry , derivative (finance) , cell , microbiology and biotechnology , electrophile , biochemistry , stereochemistry , biology , financial economics , economics , catalysis
We have developed a high‐throughput assay for screening chemical libraries for compounds that affect cell sheet migration during wound closure in epithelial cell monolayers. By using this assay, we have discovered a new inhibitor of cell sheet migration. This compound (UIC‐1005) is a 3,4‐disubstituted oxazolidinone that bears an electrophilic α , β‐unsaturated N ‐acyl group required for activity. UIC‐1005 also inhibits growth in an epithelial cell proliferation assay. The molecule does not display general toxicity at concentrations at which it potently inhibits cell sheet migration and growth. Unlike certain 3,5‐disubstituted oxazolidinones, it exhibits no antibacterial activity. UIC‐1005 therefore represents a new class of bioactive oxazolidinone derivative that may prove useful as a probe for signaling pathways leading to cell motility.