Premium
Solution‐ and Solid‐Phase Synthesis of the Polybasic Lipid‐Modified C Termini of Rho A and K‐Ras 4B
Author(s) -
Ludolph Björn,
Eisele Frank,
Waldmann Herbert
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020902)3:9<901::aid-cbic901>3.0.co;2-u
Subject(s) - linker , solid phase synthesis , chemistry , biotin , combinatorial chemistry , biot number , biochemistry , computational biology , computer science , biology , peptide , physics , mechanics , operating system
Phasing the problem : Polybasic, farnesylated, and fluorescence‐ or biotin‐tagged K‐Ras 4B ( 1 ) and Rho A peptides can be efficiently synthesized in solution or on a solid support by employment of a combination of Fmoc and Aloc urethanes with trityl groups and a trityl linker. The synthesis methods described in both solid and solution phases present new opportunities for future investigation of areas such as membrane targeting by Ras, Rho, and related lipidated proteins. Biot=biotin, Aca=aminocaproic acid.