Premium
Modification of Galactose Oxidase to Introduce Glucose 6‐Oxidase Activity
Author(s) -
Sun Lianhong,
Bulter Thomas,
Alcalde Miguel,
Petrounia Ioanna P.,
Arnold Frances H.
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020802)3:8<781::aid-cbic781>3.0.co;2-8
Subject(s) - saturated mutagenesis , glucose oxidase , galactose oxidase , biochemistry , chemistry , enzyme , mutagenesis , aldehyde oxidase , mutant , oxidase test , galactose , site directed mutagenesis , combinatorial chemistry , gene , xanthine oxidase
Combinatorial saturation mutagenesis was applied to create a mutant of the copper enzyme galactose oxidase with novel activity. The selective oxidation of the 6‐hydroxyl group of D ‐glucose to introduce an aldehyde functionality is not catalyzed by known oxidase enzymes but was achieved through mutagenesis experiments. The mutant enzyme produced is a possible starting point for the development of glucose 6‐oxidase for synthetic use in food, pharmaceuticals, and materials applications.