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Substrate Arrays as Enzyme Fingerprinting Tools
Author(s) -
Reymond JeanLouis,
Wahler Denis
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020802)3:8<701::aid-cbic701>3.0.co;2-3
Subject(s) - enzyme , enzyme assay , chemistry , chromogenic , substrate (aquarium) , fingerprint (computing) , biochemistry , combinatorial chemistry , chromatography , biology , computer science , ecology , computer security
Portrait of an enzyme : Enzyme assays are essential tools of enzymology. Profiling enzyme activities over parameter ranges such as different pH and temperature values forms the basis of mechanistic studies. The comparison of enzyme activities for different substrates delivers activity profiles that are essential for the practical use of enzymes. Fluorogenic or chromogenic substrates can be used to record enzyme activity for several substrates in parallel and the results can be displayed as 2‐dimensional arrays of gray‐scale squares that form an enzyme fingerprint. Enzymes can also be profiled by active‐site‐directed enzyme probes such as alkylating agents and the labeled enzymes can be visualized on electrophoretic gels. Enzyme profiling and fingerprinting techniques are likely to become useful in enzyme discovery and quality control applications.