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Towards the Generation of Artificial O 6 ‐Alkylguanine‐DNA Alkyltransferases: In Vitro Selection of Antibodies with Reactive Cysteine Residues
Author(s) -
Damoiseaux Robert,
Schultz Peter G.,
Johnsson Kai
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020603)3:6<573::aid-cbic573>3.0.co;2-f
Subject(s) - antibody , cysteine , phage display , in vitro , chemistry , biochemistry , dna , computational biology , combinatorial chemistry , microbiology and biotechnology , biology , enzyme , peptide , immunology
A stepwise in vitro selection scheme for the generation of artificial DNA repair proteins based on antibody scaffolds is introduced. The key to the success of the selection scheme is a careful match of the reactivity of the substrate (see scheme) with that of the antibody displayed on the phage. Antibodies with reactive cysteine residues were isolated and characterized in detail. Results showed that two cysteine residues with different reactivities play an important role in antibody folding.