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Combining Chemical and Biological Techniques to Produce Modified Proteins
Author(s) -
Goody Roger S.,
Alexandrov Kirill,
Engelhard Martin
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020503)3:5<399::aid-cbic399>3.0.co;2-u
Subject(s) - native chemical ligation , chemical biology , amino acid , peptide , chemistry , computational biology , chemical synthesis , chemical ligation , biochemistry , function (biology) , combinatorial chemistry , biology , in vitro , microbiology and biotechnology
Control over protein structure can be improved by the combination of recent developments in peptide synthesis with molecular biological and biochemical techniques. This combination makes it possible to introduce modified amino acids or other moieties with different chemical characteristics into proteins, and the technique of chemical ligation of fragments (see scheme) provides a route to larger proteins than could be synthesized in the past. Modified proteins have applications in structure–function studies and cell biology investigations and are potentially of importance for biotechnology and therapeutics.