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Cellular Uptake Studies with β ‐Peptides
Author(s) -
Rueping Magnus,
Mahajan Yogesh,
Sauer Markus,
Seebach Dieter
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020301)3:2/3<257::aid-cbic257>3.0.co;2-s
Subject(s) - cytosol , fluorescein , fluorescence microscope , fibroblast , peptide , chemistry , biochemistry , chromosomal translocation , microbiology and biotechnology , nucleus , biophysics , cellular compartment , membrane , fluorescence , cell , biology , in vitro , gene , enzyme , physics , quantum mechanics
Translocation across the membrane into cells is possible for certain peptides and proteins. To investigate the structural requirements of β ‐peptides for this process, a series of fluorescein‐labeled β ‐peptides has been synthesized and their cellular uptake into 3T3 mouse fibroblast cells determined by fluorescence microscopy (see picture). Polycationic β ‐peptides have been shown to internalize into cells, and accumulation in the cytosol and nucleus is observed.