Premium
DNA‐Directed Assembly of Bienzymic Complexes from In Vivo Biotinylated NAD(P)H:FMN Oxidoreductase and Luciferase
Author(s) -
Niemeyer Christof M.,
Koehler Joerg,
Wuerdemann Chris
Publication year - 2002
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20020301)3:2/3<242::aid-cbic242>3.0.co;2-f
Subject(s) - biotinylation , nad+ kinase , oxidoreductase , luciferase , chemistry , biosensor , streptavidin , conjugate , enzyme , schematic , biochemistry , dna , biotin , stereochemistry , combinatorial chemistry , transfection , gene , mathematical analysis , mathematics , electronic engineering , engineering
Spatially controlled assembly of enzyme conjugates can be applied to signal amplification systems for biosensors, or synthesis of novel catalysts for enzyme process technology. Artificial bienzymic systems comprised of two enzymes, NAD(P)H‐FMN oxidoreductase and luciferase (represented as blue and orange spheres, respectively, in the schematic representation) were assembled by means of DNA hybridization and streptavidin–biotin interaction.