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Kinetic Monitoring of Self‐Replicating Systems through Measurement of Fluorescence Resonance Energy Transfer
Author(s) -
Schöneborn Holger,
Bülle Jan,
von Kiedrowski Günter
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20011203)2:12<922::aid-cbic922>3.0.co;2-u
Subject(s) - förster resonance energy transfer , autocatalysis , fluorescence , kinetic energy , chemistry , oligonucleotide , microtiter plate , energy transfer , kinetics , nanotechnology , combinatorial chemistry , catalysis , biological system , materials science , chemical physics , chromatography , biochemistry , biology , physics , quantum mechanics , dna
Fluorophores Cy3 and Cy5 , when used as labels at the 5′ end of oligonucleotides, enable online kinetic monitoring of template‐directed syntheses (see scheme) and self‐replicating systems with a high density and accuracy of data. We introduce a method for the direct observation of autocatalytic growth that is based on the simultaneous formation of Cy3‐ and Cy5‐labeled template molecules. The method should be useful for the kinetic screening of catalytic cofactors in microtiter plate readers.