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Artificial Nucleases
Author(s) -
Chen Chihong B.,
Milne Lisa,
Landgraf Ralf,
Perrin David M.,
Sigman David S.
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-#
Subject(s) - computational biology , chemistry , biology
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10‐phenanthroline–copper( I ) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10‐phenanthroline–oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA‐binding proteins into site‐specific modification agents by using 1,10‐phenanthroline–copper( I ).