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In Vitro Selection of Hammerhead Ribozyme Sequence Variants
Author(s) -
Eckstein Fritz,
Kore Anilkumar R.,
Nakamaye Kay L.
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20010903)2:9<629::aid-cbic629>3.0.co;2-3
Subject(s) - hammerhead ribozyme , ribozyme , hairpin ribozyme , vs ribozyme , mammalian cpeb3 ribozyme , biology , cleavage (geology) , ligase ribozyme , stem loop , sequence (biology) , genetics , computational biology , consensus sequence , rna , base sequence , gene , paleontology , fracture (geology)
Isolation of the consensus sequence! This was the surprising result when in vitro selection was applied to the hammerhead ribozyme to obtain variants with higher cleavage rates at the 3′ side of some NUH triplets, such as GUC and AUA, and of GAC triplets. This indicates that the wild‐type sequence (see picture) has optimally evolved for catalysing these reactions. An exception was the identification of a ribozyme cleaving at the 3′ side of AUG, which is not hydrolysed by the consensus ribozyme. This ribozyme variant contains considerable changes in the core and the stem–loop II regions and is thus hammerhead‐like.