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Locating the Metal Ion in Calcium‐Binding Proteins by Using Cerium( III ) as a Probe
Author(s) -
Bertini Ivano,
Lee YongMin,
Luchinat Claudio,
Piccioli Mario,
Poggi Luisa
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20010803)2:7/8<550::aid-cbic550>3.0.co;2-t
Subject(s) - cerium , lanthanide , carboxylate , chemistry , calcium , calcium binding protein , ion , metal , metal ions in aqueous solution , crystallography , coordination sphere , inorganic chemistry , hyperfine structure , crystal structure , stereochemistry , organic chemistry , physics , quantum mechanics
The detection and assignment of NMR spectroscopic signals of carbon atoms from carbonyl and carboxylate groups in the loop hosting the Ce III ion was performed for the cerium‐substituted calcium‐binding protein calbindin D 9k . This provided a tool to characterize in solution the first coordination sphere of the metal ion. Due to the well‐documented possibility of replacing calcium with metal ions of the Ln III series, this approach turns out to be extremely efficient for characterizing in solution the coordination of calcium ions in proteins, independently of the availability of X‐ray crystal structures. The present approach completes the structural characterization of lanthanide‐substituted calcium‐binding proteins, for which the role of long‐range constraints arising from hyperfine interaction and self‐orientation has already been assessed.