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Crystal Structure of a Synthetic Cyclodecapeptide for Template‐Assembled Synthetic Protein Design
Author(s) -
Peluso Stéphane,
Rückle Thomas,
Lehmann Christian,
Mutter Manfred,
Peggion Cristina,
Crisma Marco
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20010601)2:6<432::aid-cbic432>3.0.co;2-6
Subject(s) - template , antiparallel (mathematics) , beta sheet , peptide , protein crystallization , chemistry , protein design , crystallography , nanotechnology , materials science , protein structure , combinatorial chemistry , crystallization , biochemistry , physics , organic chemistry , magnetic field , quantum mechanics
The structural prototype of a new generation of regioselectively addressable functionalized templates (RAFTs) for use in protein de novo design has been synthesized and crystallized. The structure of the aromatically substituted cyclodecapeptide was determined by X‐ray diffraction; it consists of an antiparallel β sheet spanned by heterochirally induced type II′ β turns, similar to that observed in gramicidin S. The three‐dimensional structure of the artificial template was also examined by an NMR spectroscopic analysis in solution and shown to be compatible with a β‐sheet plane suitable for accommodating secondary functional peptide fragments for the synthesis of template‐assembled synthetic proteins (TASPs).