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Selective Cleavage of Unpaired Uridines with a Tyrosine–Cyclen Conjugate
Author(s) -
Michaelis Katrin,
Kalesse Markus
Publication year - 2001
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20010105)2:1<79::aid-cbic79>3.0.co;2-6
Subject(s) - cyclen , cleave , cleavage (geology) , chemistry , phosphodiester bond , stereochemistry , conjugate , oligonucleotide , dna , combinatorial chemistry , rna , biochemistry , biology , paleontology , mathematical analysis , mathematics , fracture (geology) , gene
Sensitivity to the presence of noncanonical base pairs and dependence on the bond angles around the hydrolyzable phosphodiester linkage was demonstrated for the cleavage of RNAs by tyrosine–cyclen derivatives (cyclen = 1,4,7,10‐tetraazacyclododecane). From a small library of these conjugates compound 1 was identified to cleave preferentially at the 3′ end of unpaired uridines. To evaluate the general potential of this compound two additional aptamer RNAs (see picture) were used in the cleavage experiments. By incorporating unnatural nucleosides such as deoxyuridine and ribothymidine a more detailed picture of the cleavage mechanism was obtained.