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The Multiplasmid Approach: A New Perspective for Combinatorial Biosynthesis
Author(s) -
Hertweck Christian
Publication year - 2000
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/1439-7633(20000818)1:2<103::aid-cbic103>3.0.co;2-k
Subject(s) - computational biology , synthetic biology , drug discovery , combinatorial synthesis , biosynthesis , heterologous expression , heterologous , biology , ribosomal rna , gene , combinatorial chemistry , chemistry , biochemistry , recombinant dna
Tapping nature's vast biosynthetic capabilities in a combinatorial “mix‐and‐match” fashion has never been easier. A novel strategy permits both heterologous expression of very large biosynthetic gene clusters (→ 1 ) as well as generating large numbers of structurally diverse microbial polyketides (e. g. 2 ) and non‐ribosomal polypeptides for drug discovery.