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Evaluation of biocompatibility of the surface of polyethylene films modified with various water soluble polymers using Ar plasma‐post polymerization technique
Author(s) -
Sugiyama Kazuo,
Matsumoto Takako,
Yamazaki Yuki
Publication year - 2000
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/1439-2054(20001001)282:1<5::aid-mame5>3.0.co;2-h
Subject(s) - biocompatibility , protein adsorption , materials science , polymer , polymer chemistry , methacrylamide , polymerization , adsorption , methacrylate , copolymer , chemical engineering , surface modification , nuclear chemistry , chemistry , organic chemistry , composite material , acrylamide , engineering , metallurgy
Surface modified polyethylene (g‐PE), PMPC‐g‐PE, PGEMA‐g‐PE, PNIPAAm‐g‐PE and PHPMA‐g‐PE films with the water soluble polymers such as poly[2‐(methacryloyloxy)ethyl phosphorylcholine] (PMPC), poly[2‐(glucosyloxy)ethyl methacrylate] (PGEMA), poly( N ‐isopropylacrylamide) (PNIPAAm) and poly[ N ‐(2‐hydroxypropyl) methacrylamide] (PHPMA) were prepared by graft copolymerization using an Ar plasma‐post polymerization technique. The surface of the g‐PE films was characterized by means of X‐ray photoelectron spectroscopy and the grafting percentage of PMPC, PNIPAAm and PHPMA was found to be 5.31, 2.83, and 3.40% for the corresponding g‐PE film. Biocompatibility of the g‐PE films was evaluated by the adsorption of serum proteins and the Michaelis constant ( K m ) for the enzymatic reaction of thrombin with synthetic substrate S‐2238 in the presence of g‐PE film. The biocompatibility of water soluble polymers such as PMPC, polyoxyethylene (POE), PGEMA, PNIPAAm and PHPMA was also evaluated by the same enzymatic reaction of thrombin with S‐2238 in their polymer solutions. The K m values in the presence of water soluble polymers was found to decrease in the order PMPC > POE > PGEMA > PNIPAAm > PHPMA. As a conclusion, PMPC‐g‐PE film exhibited the most biocompatibility among g‐PE films because its surface adsorbed less protein than those of the untreated PE and other g‐PE films and it showed the largest K m for the enzymatic reaction.