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Enrichment of salmon oil with n ‐3 PUFA by lipolysis, filtration and enzymatic re‐esterification
Author(s) -
Linder Michel,
Matouba Excellent,
Fanni Jacques,
Parmentier Michel
Publication year - 2002
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/1438-9312(200208)104:8<455::aid-ejlt455>3.0.co;2-q
Subject(s) - chemistry , glyceride , lipolysis , lipase , polyunsaturated fatty acid , hydrolysis , chromatography , fish oil , differential scanning calorimetry , triacylglycerol lipase , fatty acid , organic chemistry , enzyme , biochemistry , fish <actinopterygii> , biology , physics , adipose tissue , fishery , thermodynamics
Selective enzymatic hydrolysis of salmon oil extracted without solvent from by‐products was carried out under mild conditions, using a stereospecific sn ‐1, sn ‐3 lipase Novozyme ® . A modification of the lipid class composition was obtained by controlling the degree of hydrolysis (40%, 24 h). The mixture of acylglycerols and free fatty acids was submitted to a filtration step to retain in the retentate most of the saturated fatty acids, with melting peaks ranging from ‐31.9 °C to +14.7 °C obtained by differential scanning calorimetry. This step allowed a significant increase of polyunsaturated fatty acids (PUFA) from 39.2 mol‐% in the crude oil to 43.3% in the permeate. The remaining free fatty acids in the permeate (20.2 wt‐%) was re‐esterified with an immobilized 1, 3‐specific lipase IM60. Acylglycerols synthesis reached 90% in optimized conditions. After 48 h of reaction, the distribution of monoacylglycerols, diacylglycerols and triacylglycerols was 22.1, 28.7, 43.4 (w/w), respectively. The re‐esterification step did not modify the PUFA content obtained after membrane filtration.