Open AccessImage formation in three‐photon fluorescence microscopy
Author(s) -
Sheppard C J R
Publication year - 1996
Publication title -
bioimaging
Language(s) - English
Resource type - Journals
eISSN - 1361-6374
pISSN - 0966-9051
DOI - 10.1002/1361-6374(199609)4:3<124::aid-bio2>3.0.co;2-2
Subject(s) - two photon excitation microscopy , fluorescence , fluorescence microscope , microscopy , point spread function , optics , resolution (logic) , multiphoton fluorescence microscope , fluorescence lifetime imaging microscopy , light sheet fluorescence microscopy , photon , materials science , physics , artificial intelligence , computer science
Image formation in three‐photon fluorescence microscopy is analysed. The point spread function, three‐dimensional optical transfer function and axial resolution are considered. The results are generalized to multiphoton fluorescence. Three‐photon fluorescence is particularly amenable to resolution improvement by use of pupil filters.