Open AccessMulti‐colour fluorescence imaging in connection with photodynamic therapy of δ‐amino levulinic acid (ALA) sensitised skin malignancies
Author(s) -
AnderssonEngels Stefan,
Berg Roger,
Svanberg Katarina,
Svanberg Sune
Publication year - 1995
Publication title -
bioimaging
Language(s) - English
Resource type - Journals
eISSN - 1361-6374
pISSN - 0966-9051
DOI - 10.1002/1361-6374(199509)3:3<134::aid-bio4>3.0.co;2-1
Subject(s) - photodynamic therapy , autofluorescence , fluorescence , levulinic acid , fluorescence lifetime imaging microscopy , in vivo , porphyrin , chemistry , medicine , biomedical engineering , pathology , biochemistry , optics , biology , physics , organic chemistry , microbiology and biotechnology , catalysis
A system for multi‐colour fluorescence imaging of tissue is described. The instrument is mainly developed for tissue diagnostics to identify and localise malignant tumours, but might also be useful for real‐time monitoring of the therapeutic dose delivered during photodynamic therapy. In vivo examples from various malignant skin lesions following topical δ‐amino levulinic acid (ALA) administration are presented. The diagnostic system utilises both characteristics of a fluorescent tumour marker, such as a porphyrin containing substance, and the native tissue autofluorescence to characterise the tissue. A dimensionless function of three or four simultaneously recorded fluorescence intensities is formed and an optimum‐contrast image is calculated pixel‐by‐pixel.