Open AccessInteractive 3‐D image analysis and visualization techniques for FISH‐labelled chromosomes in interphase nuclei
Author(s) -
Ikizyan Ike A,
Burde Stefan,
Leary James F
Publication year - 1994
Publication title -
bioimaging
Language(s) - English
Resource type - Journals
eISSN - 1361-6374
pISSN - 0966-9051
DOI - 10.1002/1361-6374(199403)2:1<41::aid-bio4>3.0.co;2-z
Subject(s) - visualization , autostereoscopy , computer science , computer graphics (images) , volume (thermodynamics) , interphase , confocal , computer vision , image processing , artificial intelligence , confocal microscopy , microscopy , fluorescence microscope , stereoscopy , image (mathematics) , biology , optics , physics , fluorescence , genetics , quantum mechanics
New simple and fast methods for 3‐D depth perception and interaction in volume visualization and quantitative analysis of dual‐fluorescence confocal microscopy images are presented. By integrating a 3‐D display and an associated 3‐D input device, a system has been developed which creates an autostereoscopic confocal microscopy workstation environment on an IBM‐compatible personal computer. The system incorporates appropriate 3‐D image processing algorithms and enables the user to interactively address subregions in the data volume to identify domains of interest for subsequent volume and distance measurements. In the analysis of chromosomes in interphase nuclei these capabilities are shown to be useful in finding distances between chromosome domains and their respective volumes.