Open AccessDevelopment of a method to determine workers' personal exposure levels to glyphosate
Author(s) -
Ishii Kenta,
Takeuchi Akito,
Nishinoiri Osamu,
Endo Ginji,
OnoOgasawara Mariko
Publication year - 2022
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1002/1348-9585.12345
Subject(s) - glyphosate , reproducibility , ultrapure water , detection limit , chromatography , environmental science , pulp and paper industry , chemistry , environmental engineering , microbiology and biotechnology , biology , engineering
Objectives We aimed to develop a method to determine workers' personal exposure levels to N ‐(phosphonomethyl)glycine (glyphosate) for their risk assessments. Methods The proposed method was assessed as follows: recovery, stability of samples on storage, method limit of quantification, and reproducibility. Glyphosate in air was sampled using an air‐sampling cassette containing a glass fiber filter. Ultrapure water was used to extract glyphosate from sampler filters. After derivation with 9‐fluorenylmethyloxycarbonyl chloride, samples were analyzed by high‐performance liquid chromatography using a fluorescence detector. Results Spiked samples indicated an overall recovery of 101%. After 7 days of storage at 4°C, recoveries were approximately 100%. The method limit of quantification was 0.060 μg/sample. Relative standard deviations representing overall reproducibility, defined as precision, were 1.4%–1.8%. Conclusions The method developed in this study allows 4‐h personal exposure monitoring of glyphosate at 0.250–500 μg/m 3 . Thus, this method can be used to estimate worker exposure to glyphosate.